Our technologies and expertise allow us to carry out DNA extractions and various sequencing assays for your microbial research needs.
Our expert technical staff will prepare and sequence Illumina MiSeq sequencing libraries from your samples. Our amplicon sequencing services include standard taxonomic marker-genes (16S rRNA, 18S rRNA and ITS) to identify which microbes are present and at what proportions.
We also provide sequencing of other common markers (bacterial ITS, mitochondrial 12S, rbcL, and cytochrome oxydase) and can sequence custom PCR amplicons of your gene of interest.
The ONT Nanopore and Illumina MiSeq platforms are used to generate shotgun and long-read amplicons.
Amplicon sequencing at 30,000 reads/sample MiSeq (2×250 cycles standard)
- Bacterial / Archaeal: 16S V4 or V4-V5
- Bacterial: ITS
- Fungal: ITS1
- Eukaryal: 18S V4-V5, rbcL, mitochondrial 12S/16S, and COI
- Phototrophic (e.g., algal): V8-V9
- Custom amplicon 2×150 and 2×300 also available
Our bioinformatics and software engineering expertise enable us to quickly and thoroughly analyze your genomic data, generating insightful microbiome reports.
Whether you’ve sequenced your samples with us or not, our bioinformatics team can use our optimized marker-gene and metagenomic pipelines to analyze your data. We can provide in-depth or basic analysis and reports, based on your needs and questions. Our bioinformaticians can also work with you on custom workflows, including analysis of metatranscriptome data.
- Level 1: alpha-diversity (species richness and diversity), taxonomy
- Level 2: beta-diversity (compare samples based on taxonomic composition and sample metadata)
- Level 3: further sample characterization (phylogenetic/taxonomic novelty, indicator species, mining for specific taxonomic data)
- Metagenomics: assembled genomes, gene annotation, functional enrichment, bioprospecting (discuss analysis design with analytics team)
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We can identify and track microorganisms in wastewater and highlight pathogenic species and strains that are public health concerns.
Contact us for more information.
Early Plant Pathogen Detection
Healthy Hydroponics is an innovative microbiome monitoring service dedicated to providing pathogen monitoring services to hydroponic farms and growers to enhance prediction of crop diseases and food safety.
We use PCR, high throughput sequencing technology, qPCR, machine learning, and our in-house curated pathogen database to bring you a pathogen detection and microbiome surveillance service that has been tested and improved through feedback from commercial hydroponic growers
Products
Extract high quality DNA from microbes within a wide variety of sample types. Rapid and efficient.
Our Sox Soil DNA Kit employs patent-pending methodology of bead-beating, silica spin columns, and a unique combination of chemistry, resulting in exceptionally high yields of highly purified DNA. Contaminants, including humic acids and other compounds that inhibit PCR and downstream molecular biology manipulations, are removed from the lysate. Organic solvents and proteinases are not used. The kit has been validated by successful extraction of DNA from diverse soil sample types followed by high-resolution rRNA gene and fungal internal transcribed spacer (ITS) profile analysis.
The user manual can be viewed here.
- Soil DNA Extraction Kit (Sox, 50 Samples)
Capture and preserve biomass from liquid samples intended for DNA extraction.
Our Advantages:
- Portable, it can be taken out into the field
- Simple to use, instructions can be followed by an untrained user
- No cartridge-cracking, the filter is back-washed by a solution to capture the biomass
Our Water Filtration Kit for concentrating and preserving biomass found in water samples. The preservation solution from this kit may be used directly with the Sox Soil DNA Extraction Kit.
- Water Filtration Kit (10 samples)
Coming Soon.
Interesting and new methods coming soon! Stay tuned by following us on Twitter, LinkedIn or by simply sending us an email.
Look forward to a platform tailored for you.